Hotspot

ycf3-trnS-GGA

LSC · 43705-44545 · 841 bp

Back to Glechoma

Pi 0.0048

MarkerSeek score 60.10

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 841
variable_sites: 8
pi: 0.0048
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TGCCTCCTTTTCTCCTGAAG	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.1	58.1	1000-1028 bp	1.0	74.1
ycf3-trnS-GGA_p2	GCCTCCTTTTCTCCTGAAGT	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.1	58.1	999-1027 bp	1.0	74.1
ycf3-trnS-GGA_p3	TGCCTCCTTTTCTCCTGAAG	AAGTGAAGGTGCGGAAAGAG	50.0	50.0	57.1	58.1	1031-1059 bp	1.0	73.9
ycf3-trnS-GGA_p4	GCCTCCTTTTCTCCTGAAGT	AAGTGAAGGTGCGGAAAGAG	50.0	50.0	57.1	58.1	1030-1058 bp	1.0	73.9
ycf3-trnS-GGA_p5	TGCCTCCTTTTCTCCTGAAG	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.1	57.8	971-999 bp	1.0	73.4