Hotspot

atpA-atpF

LSC · 11383-11495 · 113 bp

Back to Glechoma

Pi 0.0144

MarkerSeek score 62.70

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 113
variable_sites: 2
pi: 0.0144
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CCTACTTGAAGTACGGTGCC	AAGGAGCTCTAGGAACCCTG	55.0	55.0	58.0	58.1	309-327 bp	1.0	83.4
atpA-atpF_p2	CCTACTTGAAGTACGGTGCC	CAAGGAGCTCTAGGAACCCT	55.0	55.0	58.0	58.1	310-328 bp	1.0	83.4
atpA-atpF_p3	CCTACTTGAAGTACGGTGCC	GGAGCTCTAGGAACCCTGAA	55.0	55.0	58.0	58.1	307-325 bp	1.0	83.4
atpA-atpF_p4	CCTACTTGAAGTACGGTGCC	GGCATGTTGGGAGTGATGAA	55.0	50.0	58.0	58.2	238-256 bp	1.0	83.3
atpA-atpF_p5	GTCGCCTACTTGAAGTACGG	AAGGAGCTCTAGGAACCCTG	55.0	55.0	58.1	58.1	313-331 bp	1.0	82.7