Hotspot

rps16-trnQ-UUG

LSC · 5711-6645 · 935 bp

Back to Glechoma

Pi 0.00713

MarkerSeek score 57.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 935
variable_sites: 13
pi: 0.00713
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CAACAGGTCGTGTCCTTCAA	GAAATTGAAATGGGGCGTGG	50.0	50.0	58.1	58.0	1084-1173 bp	1.0	82.5
rps16-trnQ-UUG_p2	CAACAGGTCGTGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.1	58.1	1026-1115 bp	1.0	82.1
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GAAATTGAAATGGGGCGTGG	45.0	50.0	58.1	58.0	1066-1155 bp	1.0	81.8
rps16-trnQ-UUG_p4	CAACAGGTCGTGTCCTTCAA	GTCTCGCTATTCGGAGGTTC	50.0	55.0	58.1	57.9	1039-1128 bp	1.0	81.7
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1008-1097 bp	1.0	81.4