Hotspot

ycf1

SSC · 120976-126387 · 5412 bp

Back to Gladiolus

Pi 0.37

MarkerSeek score 74.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5412
variable_sites: 1654
pi: 0.37
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGTTCTGTCTCCTTTCCCCA	AAAAAGGTCAGAAGCCTGGG	50.0	50.0	57.9	58.0	405 bp	0.5	59.0
ycf1_p2	AGTTCTGTCTCCTTTCCCCA	CGCACAACAATCCGACTTTC	50.0	50.0	57.9	58.0	699 bp	0.5	58.7
ycf1_p3	GCAGTTCTGTCTCCTTTCCC	AAAAAGGTCAGAAGCCTGGG	55.0	50.0	58.2	58.0	407 bp	0.5	58.5
ycf1_p4	GGCAGTTCTGTCTCCTTTCC	AAAAAGGTCAGAAGCCTGGG	55.0	50.0	58.2	58.0	408 bp	0.5	58.5
ycf1_p5	GGGCAGTTCTGTCTCCTTTC	AAAAAGGTCAGAAGCCTGGG	55.0	50.0	58.2	58.0	409 bp	0.5	58.5