Hotspot

psaC

SSC · 114315-114560 · 246 bp

Back to Gladiolus

Pi 0.482

MarkerSeek score 70.40

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 80
pi: 0.482
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.67
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	GATTGACCAAAAAGCCCGTG	ATCACAAGCCGAAACATGGT	50.0	45.0	57.9	58.1	938 bp	0.5	58.8
psaC_p2	CCCCCTGCAGTGTACAAAAT	ATCACAAGCCGAAACATGGT	50.0	45.0	58.1	58.1	1476 bp	0.5	58.7
psaC_p3	GATTGACCAAAAAGCCCGTG	GCGATTGGGCTAGCTATTGT	50.0	50.0	57.9	58.1	779 bp	0.5	58.6
psaC_p4	GATTGACCAAAAAGCCCGTG	AGTCCCTTGGTCCTTTCTCA	50.0	50.0	57.9	57.9	521 bp	0.5	58.5
psaC_p5	CCCCCTGCAGTGTACAAAAT	GCGATTGGGCTAGCTATTGT	50.0	50.0	58.1	58.1	1317 bp	0.5	58.5