Hotspot

ndhG

SSC · 115378-115908 · 531 bp

Back to Gladiolus

Pi 0.464

MarkerSeek score 78.30

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 200
pi: 0.464
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	ACCATGTTTCGGCTTGTGAT	TACATCAGAGCGTTTCCGTG	45.0	50.0	58.1	58.0	1422 bp	0.5	59.2
ndhG_p2	ACCATGTTTCGGCTTGTGAT	AACAATACGAGCTGCACGAT	45.0	45.0	58.1	58.0	1524 bp	0.5	59.2
ndhG_p3	ACAATAGCTAGCCCAATCGC	TACATCAGAGCGTTTCCGTG	50.0	50.0	58.1	58.0	1581 bp	0.5	59.0
ndhG_p4	ACAATAGCTAGCCCAATCGC	AACAATACGAGCTGCACGAT	50.0	45.0	58.1	58.0	1683 bp	0.5	59.0
ndhG_p5	AATAGCTAGCCCAATCGCTG	TACATCAGAGCGTTTCCGTG	50.0	50.0	57.8	58.0	1579 bp	0.5	58.5