Hotspot

ndhF

SSC · 107622-109838 · 2217 bp

Back to Gladiolus

Pi 0.476

MarkerSeek score 81.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2217
variable_sites: 984
pi: 0.476
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGAATGCCGTCGCCTATTTT	GATGCATGCTACGATCCGTA	45.0	50.0	58.0	57.7	2838 bp	0.5	56.9
ndhF_p2	GCCGTCGCCTATTTTCACTA	GATGCATGCTACGATCCGTA	50.0	50.0	58.1	57.7	2833 bp	0.5	56.7
ndhF_p3	AGAATGCCGTCGCCTATTTT	TTGATGCATGCTACGATCCG	45.0	50.0	58.0	58.5	2840 bp	0.5	55.7
ndhF_p4	GCCGTCGCCTATTTTCACTA	TTGATGCATGCTACGATCCG	50.0	50.0	58.1	58.5	2835 bp	0.5	55.5
ndhF_p5	AATGCCGTCGCCTATTTTCA	GATGCATGCTACGATCCGTA	45.0	50.0	58.3	57.7	2836 bp	0.5	55.2