Hotspot

ndhE

SSC · 114902-115207 · 306 bp

Back to Gladiolus

Pi 0.485

MarkerSeek score 75.80

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 132
pi: 0.485
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	ACCCATGCTTCGAGTTGTTT	AGGGCTGGGGGTAGTATTAC	45.0	55.0	58.0	57.9	1517 bp	0.5	58.9
ndhE_p2	ACCCATGCTTCGAGTTGTTT	GGGCTGGGGGTAGTATTACT	45.0	55.0	58.0	57.9	1516 bp	0.5	58.9
ndhE_p3	GCTTCGAGTTGTTTCATGCC	AGGGCTGGGGGTAGTATTAC	50.0	55.0	58.0	57.9	1511 bp	0.5	58.8
ndhE_p4	GCTTCGAGTTGTTTCATGCC	GGGCTGGGGGTAGTATTACT	50.0	55.0	58.0	57.9	1510 bp	0.5	58.8
ndhE_p5	GATTGACCAAAAAGCCCGTG	GGGCTGGGGGTAGTATTACT	50.0	55.0	57.9	57.9	1636 bp	0.5	58.6