Hotspot

ndhD

SSC · 112687-114192 · 1506 bp

Back to Gladiolus

Pi 0.483

MarkerSeek score 77.30

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1506
variable_sites: 529
pi: 0.483
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GAGGCATGGGGGTCTTATTG	AAACAACTCGAAGCATGGGT	55.0	45.0	58.0	58.0	2121 bp	0.5	59.7
ndhD_p2	CGTGCGAGTTTTTGAGAACC	AAACAACTCGAAGCATGGGT	50.0	45.0	58.0	58.0	1838 bp	0.5	59.7
ndhD_p3	GGTTCGTGCGAGTTTTTGAG	AAACAACTCGAAGCATGGGT	50.0	45.0	58.0	58.0	1842 bp	0.5	59.7
ndhD_p4	GAGGCATGGGGGTCTTATTG	GGCATGAAACAACTCGAAGC	55.0	50.0	58.0	58.0	2127 bp	0.5	59.6
ndhD_p5	CGTGCGAGTTTTTGAGAACC	GGCATGAAACAACTCGAAGC	50.0	50.0	58.0	58.0	1844 bp	0.5	59.6