Hotspot

ndhA

SSC · 116784-118956 · 2173 bp

Back to Gladiolus

Pi 0.476

MarkerSeek score 80.90

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2173
variable_sites: 933
pi: 0.476
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ATCGTGCAGCTCGTATTGTT	GGGCATTATTGGTGGAGAGG	45.0	55.0	58.0	58.0	2894 bp	0.5	59.8
ndhA_p2	ATCGTGCAGCTCGTATTGTT	GGGGCTTATCAGGACCAATG	45.0	55.0	58.0	58.0	2862 bp	0.5	59.8
ndhA_p3	ACCAGTCACCATAGGGAACA	GGGCATTATTGGTGGAGAGG	50.0	55.0	58.0	58.0	2854 bp	0.5	59.6
ndhA_p4	ACCAGTCACCATAGGGAACA	GGGGCTTATCAGGACCAATG	50.0	55.0	58.0	58.0	2822 bp	0.5	59.6
ndhA_p5	ATCGTGCAGCTCGTATTGTT	CGGCGAAATGAGAGAATCCA	45.0	50.0	58.0	58.1	2717 bp	0.5	59.4