Hotspot

rpl32-trnL-UAG

SSC · 110544-111332 · 789 bp

Back to Gladiolus

Pi 0.458
MarkerSeek score 79.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
789
variable_sites
290
pi
0.458
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.80
markerseek_score
79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TACGGATCGTAGCATGCATC AGCGTGTCTACCGATTTCAC 50.0 50.0 57.7 58.0 1119 bp 0.5 57.2
rpl32-trnL-UAG_p2 TACGGATCGTAGCATGCATC CAGCGTGTCTACCGATTTCA 50.0 50.0 57.7 58.0 1120 bp 0.5 57.1
rpl32-trnL-UAG_p3 TACGGATCGTAGCATGCATC TGGCGAAACCTTGGGTAAAA 50.0 45.0 57.7 57.9 1597 bp 0.5 56.7
rpl32-trnL-UAG_p4 TACGGATCGTAGCATGCATC TCGAAGAAATCCAACGGGTC 50.0 50.0 57.7 57.9 1447 bp 0.5 56.5
rpl32-trnL-UAG_p5 TACGGATCGTAGCATGCATC TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.7 57.9 1129 bp 0.5 56.2