Hotspot

ycf1

SSC · 121373-127018 · 5646 bp

Back to Gironniera

Pi 0.343

MarkerSeek score 75.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5646
variable_sites: 1725
pi: 0.343
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AATTTCCCGAATGGTCCGAG	AGTTGTTTTCGGGGAGAACC	50.0	50.0	58.0	58.0	295 bp	0.5	59.5
ycf1_p2	GACGATTTGGAACACGAGGA	AGTTGTTTTCGGGGAGAACC	50.0	50.0	57.9	58.0	908 bp	0.5	59.2
ycf1_p3	ACGATTTGGAACACGAGGAG	AGTTGTTTTCGGGGAGAACC	50.0	50.0	57.9	58.0	907 bp	0.5	59.2
ycf1_p4	TCAAGCAAACGTACACTCCC	AGTTGTTTTCGGGGAGAACC	50.0	50.0	58.1	58.0	642 bp	0.5	58.8
ycf1_p5	AGGAGGGAGAAATCGACGAA	AGTTGTTTTCGGGGAGAACC	50.0	50.0	58.2	58.0	892 bp	0.5	58.6