Hotspot

rpl32

SSC · 137738-137896 · 159 bp

Back to Gironniera

Pi 0.432

MarkerSeek score 75.20

Primer available yes

Label rpl32

Feature Metadata

label_name: rpl32
feature_type: gene
region: SSC
length_bp: 159
variable_sites: 60
pi: 0.432
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32_p1	TCGTCGCCAGTGAAGTAGTA	ATTCGATGGACTCTGTTGGA	50.0	45.0	58.2	56.3	1461 bp	0.5	44.5
rpl32_p2	TCGTCGCCAGTGAAGTAGTA	TCGATGGACTCTGTTGGATT	50.0	45.0	58.2	56.3	1459 bp	0.5	44.5
rpl32_p3	TCGTCGCCAGTGAAGTAGTA	TTCGATGGACTCTGTTGGAT	50.0	45.0	58.2	56.3	1460 bp	0.5	44.5
rpl32_p4	TCGTCGCCAGTGAAGTAGTA	AGGAAACGCTATGATCTCCA	50.0	45.0	58.2	56.0	1627 bp	0.5	42.7
rpl32_p5	TCGTCGCCAGTGAAGTAGTA	TCGATGGACTCTGTTGGATTT	50.0	42.9	58.2	57.0	1459 bp	0.5	42.6