Hotspot

ndhI

SSC · 131390-131893 · 504 bp

Back to Gironniera

Pi 0.486

MarkerSeek score 79.80

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 504
variable_sites: 216
pi: 0.486
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	ACTTGGGCGGTTGGAATATC	ACCAGCAAAGTAACCCCATC	50.0	50.0	57.9	57.8	1546 bp	0.5	57.9
ndhI_p2	ACTTGGGCGGTTGGAATATC	AGGGTGTGAATTAATGCGGG	50.0	50.0	57.9	58.2	1115 bp	0.5	57.7
ndhI_p3	ACAGTTCTTTACTTGGGCGG	ACCAGCAAAGTAACCCCATC	50.0	50.0	58.1	57.8	1556 bp	0.5	57.4
ndhI_p4	CAGTTCTTTACTTGGGCGGT	ACCAGCAAAGTAACCCCATC	50.0	50.0	58.1	57.8	1555 bp	0.5	57.4
ndhI_p5	GGTTCTGGAATAGCGATGGG	ACCAGCAAAGTAACCCCATC	55.0	50.0	58.1	57.8	1863 bp	0.5	57.3