Hotspot

ndhH

SSC · 127815-128996 · 1182 bp

Back to Gironniera

Pi 0.475

MarkerSeek score 82.40

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 509
pi: 0.475
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	CGCCAAAGGATGCTGTCTTA	ACAAGGTGAGAGCCAAAAGG	50.0	50.0	58.3	58.0	1751 bp	0.5	57.8
ndhH_p2	CGCCAAAGGATGCTGTCTTA	GTTGTTGTATCCCTGCCGAT	50.0	50.0	58.3	58.0	1564 bp	0.5	57.6
ndhH_p3	TTGGGAAAACGCCAAAGGAT	ACAAGGTGAGAGCCAAAAGG	45.0	50.0	58.3	58.0	1760 bp	0.5	57.6
ndhH_p4	GAAAACGCCAAAGGATGCTG	ACAAGGTGAGAGCCAAAAGG	50.0	50.0	58.3	58.0	1756 bp	0.5	57.5
ndhH_p5	CGCCAAAGGATGCTGTCTTA	TGTCTTAACGAATCGCACGT	50.0	45.0	58.3	57.9	1954 bp	0.5	57.5