Hotspot

ndhG

SSC · 132277-132807 · 531 bp

Back to Gironniera

Pi 0.459

MarkerSeek score 81.10

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 222
pi: 0.459
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	ACCCGTCGTTGATTGGAAAT	TCGGATCGAGTCATGTGAGA	45.0	50.0	57.8	58.0	1881 bp	0.5	58.1
ndhG_p2	CCCGCATTAATTCACACCCT	TCGGATCGAGTCATGTGAGA	50.0	50.0	58.2	58.0	1370 bp	0.5	57.8
ndhG_p3	AAATTGCCCTGGGTCGTTTA	TCGGATCGAGTCATGTGAGA	45.0	50.0	57.7	58.0	1699 bp	0.5	57.3
ndhG_p4	CTACCCGTCGTTGATTGGAA	TCGGATCGAGTCATGTGAGA	50.0	50.0	57.6	58.0	1883 bp	0.5	56.7
ndhG_p5	GTCCAATAACTGTACCCGCA	TCGGATCGAGTCATGTGAGA	50.0	50.0	57.6	58.0	1384 bp	0.5	56.6