Hotspot

ndhA

SSC · 128998-131307 · 2310 bp

Back to Gironniera

Pi 0.466

MarkerSeek score 77.70

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2310
variable_sites: 855
pi: 0.466
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TAGGGGATCAGAACGGGTTT	ATTTCCAATCAACGACGGGT	50.0	45.0	58.0	57.8	2804 bp	0.5	58.1
ndhA_p2	TAGGGGATCAGAACGGGTTT	TAAACGACCCAGGGCAATTT	50.0	45.0	58.0	57.7	2986 bp	0.5	57.3
ndhA_p3	ATAGGGGATCAGAACGGGTT	ATTTCCAATCAACGACGGGT	50.0	45.0	57.8	57.8	2805 bp	0.5	56.9
ndhA_p4	TCTAGAAGGAATTCCGGGGG	ATTTCCAATCAACGACGGGT	55.0	45.0	58.2	57.8	2995 bp	0.5	56.9
ndhA_p5	AACGGGTTTCCTTGGAGATG	ATTTCCAATCAACGACGGGT	50.0	45.0	57.8	57.8	2793 bp	0.5	56.8