Hotspot

ccsA

SSC · 135773-136738 · 966 bp

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Pi 0.46

MarkerSeek score 79.30

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 966
variable_sites: 355
pi: 0.46
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	CTTCGCGATGGATTACACGA	GTGAAATCGGTAGACACGCT	50.0	50.0	58.2	58.0	1250 bp	0.5	58.6
ccsA_p2	TCGCGATGGATTACACGAAG	GTGAAATCGGTAGACACGCT	50.0	50.0	58.2	58.0	1248 bp	0.5	58.6
ccsA_p3	CTTCGCGATGGATTACACGA	TGAAATCGGTAGACACGCTG	50.0	50.0	58.2	58.0	1249 bp	0.5	58.6
ccsA_p4	TCGCGATGGATTACACGAAG	TACTTCACTGGCGACGATTG	50.0	50.0	58.2	58.0	1316 bp	0.5	58.6
ccsA_p5	TCGCGATGGATTACACGAAG	TGAAATCGGTAGACACGCTG	50.0	50.0	58.2	58.0	1247 bp	0.5	58.6