Hotspot

trnL-UAG-rpl32

SSC · 136947-137737 · 791 bp

Back to Gironniera

Pi 0.403
MarkerSeek score 79.30
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
791
variable_sites
255
pi
0.403
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.80
markerseek_score
79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 TGGCGCGGTTATTACGTTTA ATTCGATGGACTCTGTTGGA 45.0 45.0 58.0 56.3 2029 bp 0.5 46.0
trnL-UAG-rpl32_p2 TGGCGCGGTTATTACGTTTA TCGATGGACTCTGTTGGATT 45.0 45.0 58.0 56.3 2027 bp 0.5 46.0
trnL-UAG-rpl32_p3 TGGCGCGGTTATTACGTTTA TTCGATGGACTCTGTTGGAT 45.0 45.0 58.0 56.3 2028 bp 0.5 46.0
trnL-UAG-rpl32_p4 AGCGTGTCTACCGATTTCAC TCGATGGACTCTGTTGGATT 50.0 45.0 58.0 56.3 1530 bp 0.5 46.0
trnL-UAG-rpl32_p5 AGCGTGTCTACCGATTTCAC TTCGATGGACTCTGTTGGAT 50.0 45.0 58.0 56.3 1531 bp 0.5 46.0