Hotspot

ycf1-rps15

SSC · 127019-127437 · 419 bp

Back to Gironniera

Pi 0.455

MarkerSeek score 78.60

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 419
variable_sites: 172
pi: 0.455
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	ATGGAAGCGGGGTTTGATTT	GATGAGTCGAAGAACGCCAT	45.0	50.0	58.1	58.1	1624 bp	0.5	58.9
ycf1-rps15_p2	ATGGAAGCGGGGTTTGATTT	CTTCACCGTCGAGAGTAACG	45.0	55.0	58.1	58.1	1643 bp	0.5	58.5
ycf1-rps15_p3	ATGGAAGCGGGGTTTGATTT	GGACCGAGCCATAACAGATG	45.0	55.0	58.1	58.1	1896 bp	0.5	58.5
ycf1-rps15_p4	ATGGAAGCGGGGTTTGATTT	ACGGTTATTGCTTCGGTGAA	45.0	45.0	58.1	57.8	1785 bp	0.5	58.1
ycf1-rps15_p5	GCCCCTAGACGTCGTATAGA	GATGAGTCGAAGAACGCCAT	55.0	50.0	57.8	58.1	1162 bp	0.5	58.0