Hotspot

rps15-ycf1

SSC · 121868-122257 · 390 bp

Back to Girardinia

Pi 0.0397

MarkerSeek score 75.60

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 390
variable_sites: 39
pi: 0.0397
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CGGAGGCCTCTCTGAGATAA	GCAGTCGTTTCAGTCTGCTA	55.0	50.0	58.0	57.9	564-604 bp	1.0	89.2
rps15-ycf1_p2	CGGAGGCCTCTCTGAGATAA	TGTCTCCTGCTTAGACTGCT	55.0	50.0	58.0	58.1	1162-1198 bp	1.0	86.9
rps15-ycf1_p3	CGGAGGCCTCTCTGAGATAA	TTATCCCCTAGGCGTCGTAG	55.0	55.0	58.0	58.1	805-841 bp	0.833333	81.3
rps15-ycf1_p4	CGGAGGCCTCTCTGAGATAA	AGGTCCCTAGAAAAAGGCCT	55.0	50.0	58.0	57.9	959 bp	0.166667	46.1
rps15-ycf1_p5	ATGAGTCGAAGAACACCGTG	AGGTCCCTAGAAAAAGGCCT	50.0	50.0	57.9	57.9	1266 bp	0.166667	45.6