Hotspot

ndhF-rpl32

SSC · 111044-111860 · 817 bp

Back to Girardinia

Pi 0.023

MarkerSeek score 70.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 817
variable_sites: 49
pi: 0.023
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACTGGAAGGGGAATAAAAGGT	TCCAAAGATTTTTACGAATACG	42.9	31.8	56.5	52.9	892-931 bp	1.0	48.4
ndhF-rpl32_p2	AGGAACTGGAAGGGGAATAA	TCCAAAGATTTTTACGAATACG	45.0	31.8	55.4	52.9	896-935 bp	1.0	48.3
ndhF-rpl32_p3	ACTGGAAGGGGAATAAAAGGT	TTCCAAAGATTTTTACGAATACG	42.9	30.4	56.5	53.8	893-932 bp	1.0	48.3
ndhF-rpl32_p4	ACTGGAAGGGGAATAAAAGGT	CCTTTCTTTTTCCAAAGATTTTTACGA	42.9	29.6	56.5	57.7	902-941 bp	1.0	48.3
ndhF-rpl32_p5	AACTGGAAGGGGAATAAAAGGT	TCCAAAGATTTTTACGAATACG	40.9	31.8	57.2	52.9	893-932 bp	1.0	48.3