Hotspot

trnL-UAA-trnF-GAA

LSC · 47290-47782 · 493 bp

Back to Girardinia

Pi 0.0322

MarkerSeek score 62.30

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 493
variable_sites: 42
pi: 0.0322
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.5	58.0	540-736 bp	1.0	83.1
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	576-772 bp	1.0	82.3
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	GTGCCAGGAACCAGATTTGA	50.0	50.0	57.5	58.1	591-787 bp	1.0	81.9
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	TGTGCCAGGAACCAGATTTG	50.0	50.0	57.5	58.4	592-788 bp	1.0	79.5
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	573-769 bp	1.0	79.1