Hotspot

psbM-trnD-GUC

LSC · 29184-29663 · 480 bp

Back to Girardinia

Pi 0.0256

MarkerSeek score 62.20

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 480
variable_sites: 34
pi: 0.0256
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TAGTAAGTCTCGCTTGGGCT	CAAAAATTCGCCGATCCACC	50.0	50.0	58.2	58.1	1669-1688 bp	0.833333	76.0
psbM-trnD-GUC_p2	TCGGACGAGTCAAAACTGTC	CAAAAATTCGCCGATCCACC	50.0	50.0	57.9	58.1	1412 bp	0.166667	44.9
psbM-trnD-GUC_p3	TCGGACGAGTCAAAACTGTC	CCAAAAATTCGCCGATCCAC	50.0	50.0	57.9	58.1	1413 bp	0.166667	44.9
psbM-trnD-GUC_p4	CGGACGAGTCAAAACTGTCT	CAAAAATTCGCCGATCCACC	50.0	50.0	57.9	58.1	1411 bp	0.166667	44.8
psbM-trnD-GUC_p5	CGGACGAGTCAAAACTGTCT	CCAAAAATTCGCCGATCCAC	50.0	50.0	57.9	58.1	1412 bp	0.166667	44.8