Hotspot

petN-psbM

LSC · 28537-29078 · 542 bp

Back to Girardinia

Pi 0.0283

MarkerSeek score 58.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 542
variable_sites: 38
pi: 0.0283
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGTTCATTCTAGTCCCTACTGC	50.0	45.5	58.2	57.3	693-698 bp	1.0	61.9
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	TGTTCATTCTAGTCCCTACTGC	50.0	45.5	57.6	57.3	632-637 bp	1.0	60.8
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	GTTCATTCTAGTCCCTACTGCT	50.0	45.5	58.2	57.0	692-697 bp	1.0	59.8
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	GTTCATTCTAGTCCCTACTGCT	50.0	45.5	57.6	57.0	631-636 bp	1.0	58.6
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	GTTCATTCTAGTCCCTACTGCTT	50.0	43.5	58.2	57.7	692-697 bp	1.0	57.1