Hotspot

atpH-atpI

LSC · 13519-14131 · 613 bp

Back to Girardinia

Pi 0.0269

MarkerSeek score 61.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 613
variable_sites: 40
pi: 0.0269
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CCAGCGGTATTCAAGCTCTT	45.0	50.0	58.0	58.0	657-722 bp	1.0	88.4
atpH-atpI_p2	AAAAAGCAAAAGCAGCAGCA	CCAGCGGTATTCAAGCTCTT	40.0	50.0	58.0	58.0	941-1006 bp	1.0	87.4
atpH-atpI_p3	AAAAGCAAAAGCAGCAGCAA	CCAGCGGTATTCAAGCTCTT	40.0	50.0	58.0	58.0	940-1005 bp	1.0	87.4
atpH-atpI_p4	AAAGCAAAAGCAGCAGCAAA	CCAGCGGTATTCAAGCTCTT	40.0	50.0	58.0	58.0	939-1004 bp	1.0	87.4
atpH-atpI_p5	GCGGATGGCCAATAACCTAA	CCAGCGGTATTCAAGCTCTT	50.0	50.0	58.0	58.0	1375-1437 bp	1.0	86.9