Hotspot

psbK-psbI

LSC · 7295-7770 · 476 bp

Back to Girardinia

Pi 0.0263

MarkerSeek score 70.50

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 476
variable_sites: 32
pi: 0.0263
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CAAGCCGCTGTAAGTTTTCG	TCCGAAGATGAATAAAGAGACA	50.0	36.4	58.0	54.7	550-566 bp	1.0	46.9
psbK-psbI_p2	CAAGCCGCTGTAAGTTTTCG	AGGAATCCGAAGATGAATAAAGAGA	50.0	36.0	58.0	57.6	555-571 bp	1.0	46.9
psbK-psbI_p3	CAAGCCGCTGTAAGTTTTCG	GGAATCCGAAGATGAATAAAGAGAC	50.0	40.0	58.0	57.6	554-570 bp	1.0	46.9
psbK-psbI_p4	CAAGCCGCTGTAAGTTTTCG	AGATAGGAATCCGAAGATGA	50.0	40.0	58.0	52.6	559-575 bp	1.0	46.8
psbK-psbI_p5	CAAGCCGCTGTAAGTTTTCG	GGATCATTAGATAGGAATCCGAAGA	50.0	40.0	58.0	57.6	567-583 bp	1.0	46.7