Hotspot

atpH-atpI

LSC · 14240-15371 · 1132 bp

Back to Gillenia

Pi 0.0168
MarkerSeek score 68.70
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1132
variable_sites
19
pi
0.0168
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 AATAGAAGCAAGCCCGACAG GCGAATCCATGGAAGGTCAT 50.0 50.0 58.0 58.0 1224-1227 bp 1.0 81.0
atpH-atpI_p2 ATAACGGAAGCGGCAGAAAT GCGAATCCATGGAAGGTCAT 45.0 50.0 58.0 58.0 1190-1193 bp 1.0 81.0
atpH-atpI_p3 AATAGAAGCAAGCCCGACAG TTTTTGCAACCTTAGCTGCG 50.0 45.0 58.0 57.9 1254-1257 bp 1.0 80.1
atpH-atpI_p4 ATAACGGAAGCGGCAGAAAT TTTTTGCAACCTTAGCTGCG 45.0 45.0 58.0 57.9 1220-1223 bp 1.0 80.1
atpH-atpI_p5 AATAACGGAAGCGGCAGAAA GCGAATCCATGGAAGGTCAT 45.0 50.0 58.2 58.0 1191-1194 bp 1.0 79.8