Hotspot

trnR-UCU-atpA

LSC · 10124-10646 · 523 bp

Back to Gillenia

Pi 0.0299

MarkerSeek score 65.30

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 523
variable_sites: 15
pi: 0.0299
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	AGACATTTACCGACGAAGCG	47.6	50.0	57.3	58.4	616-631 bp	1.0	68.2
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	GGAAGCCCTTTTGAAAGAAGC	47.6	47.6	57.3	58.0	597-612 bp	1.0	63.1
trnR-UCU-atpA_p3	GTTCAAATCCTATTGGACGCA	AGACATTTACCGACGAAGCG	42.9	50.0	56.6	58.4	615-630 bp	1.0	62.6
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	ACCAAGACATTTACCGACGA	47.6	45.0	57.3	56.9	620-635 bp	1.0	62.3
trnR-UCU-atpA_p5	GGTTCAAATCCTATTGGACGC	TCAGGAACAGATGGAACGTT	47.6	45.0	57.3	56.8	573-588 bp	1.0	61.8