Hotspot

trnK-UUU-rps16

LSC · 4412-5186 · 775 bp

Back to Gillenia

Pi 0.0251

MarkerSeek score 64.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 775
variable_sites: 19
pi: 0.0251
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	GGGTTTTTACGGAACTTCGC	50.0	50.0	58.2	57.7	856-869 bp	1.0	79.6
trnK-UUU-rps16_p2	AAAGCCGAGTACTCTACCGT	GGTTTTTACGGAACTTCGCC	50.0	50.0	58.2	57.7	855-868 bp	1.0	79.6
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	GGGTTTTTACGGAACTTCGC	50.0	50.0	58.2	57.7	855-868 bp	1.0	79.6
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	GGTTTTTACGGAACTTCGCC	50.0	50.0	58.2	57.7	854-867 bp	1.0	79.6
trnK-UUU-rps16_p5	CCGCACTTAAAAGCCGAGTA	GGTTTTTACGGAACTTCGCC	50.0	50.0	58.3	57.7	864-877 bp	1.0	78.7