Hotspot

ndhF

SSC · 113982-116213 · 2232 bp

Back to Gilbertiodendron

Pi 0.0114

MarkerSeek score 50.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 38
pi: 0.0114
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGGGCCCTCTTATCTCTTCC	TTTCACTTCGTGCTTTGCAA	55.0	40.0	57.9	57.1	2953-2979 bp	1.0	73.9
ndhF_p2	AGGGCCCTCTTATCTCTTCC	TTTTCACTTCGTGCTTTGCA	55.0	40.0	57.9	57.1	2954-2980 bp	1.0	73.9
ndhF_p3	GGGCCCTCTTATCTCTTCCT	TTTCACTTCGTGCTTTGCAA	55.0	40.0	57.9	57.1	2952-2978 bp	1.0	73.9
ndhF_p4	GGGCCCTCTTATCTCTTCCT	TTTTCACTTCGTGCTTTGCA	55.0	40.0	57.9	57.1	2953-2979 bp	1.0	73.9
ndhF_p5	TCGGAGCTCAGGTTATGGAA	TTTCACTTCGTGCTTTGCAA	50.0	40.0	58.1	57.1	2930-2956 bp	1.0	73.7