Hotspot

ccsA-ndhD

SSC · 119675-119972 · 298 bp

Back to Gilbertiodendron

Pi 0.0327

MarkerSeek score 75.30

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 298
variable_sites: 12
pi: 0.0327
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGGGCTTTCTTATAATTTGGGT	CAGTTGACAAGGTCGAAGCT	36.4	50.0	55.6	58.1	400-428 bp	1.0	50.4
ccsA-ndhD_p2	TGGGCTTTCTTATAATTTGGGT	TCAGTTGACAAGGTCGAAGC	36.4	50.0	55.6	58.1	401-429 bp	1.0	50.4
ccsA-ndhD_p3	ATGGGCTTTCTTATAATTTGGGT	CAGTTGACAAGGTCGAAGCT	34.8	50.0	56.2	58.1	401-429 bp	1.0	46.7
ccsA-ndhD_p4	ATGGGCTTTCTTATAATTTGGGT	TCAGTTGACAAGGTCGAAGC	34.8	50.0	56.2	58.1	402-430 bp	1.0	46.6
ccsA-ndhD_p5	GGTGTAAATTCTTCCATAGTAGCC	CAGTTGACAAGGTCGAAGCT	41.7	50.0	57.1	58.1	428-456 bp	1.0	46.3