Hotspot

petA-psbJ

LSC · 65777-66636 · 860 bp

Back to Gilbertiodendron

Pi 0.0239

MarkerSeek score 64.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 860
variable_sites: 29
pi: 0.0239
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TCCGGGACCAGAACTTTTTG	TTTACAGTACGATGGCTGGC	50.0	50.0	58.0	58.0	1592-1612 bp	1.0	83.6
petA-psbJ_p2	TCCGGGACCAGAACTTTTTG	AACTGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	1683-1703 bp	1.0	83.5
petA-psbJ_p3	TCCGGGACCAGAACTTTTTG	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1682-1702 bp	1.0	83.5
petA-psbJ_p4	TCCGGGACCAGAACTTTTTG	GATCTTTTTAGGAGGCGCCA	50.0	50.0	58.0	58.0	1639-1659 bp	1.0	83.3
petA-psbJ_p5	TCCGGGACCAGAACTTTTTG	TGCGGGTTGGTTATTTGTCA	50.0	45.0	58.0	58.0	1785-1805 bp	1.0	83.1