Hotspot

trnG-UCC-trnfM-CAU

LSC · 39576-39748 · 173 bp

Back to Gilbertiodendron

Pi 0.0529

MarkerSeek score 74.20

Primer available yes

Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name: trnG-UCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 173
variable_sites: 9
pi: 0.0529
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	211-251 bp	1.0	85.5
trnG-UCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	TGAGGTCACGGGTTCAAATC	50.0	50.0	57.8	57.8	209-249 bp	1.0	84.4
trnG-UCC-trnfM-CAU_p3	GGGTTCAATTCCCGCTATCC	CTTGAGGTCACGGGTTCAAA	55.0	50.0	58.4	58.0	190-230 bp	1.0	84.1
trnG-UCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	GTAGCTCGCAAGGCTCATAA	50.0	50.0	57.8	57.8	232-272 bp	1.0	83.8
trnG-UCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	TAGCTCGCAAGGCTCATAAC	50.0	50.0	57.8	57.8	231-271 bp	1.0	83.8