Hotspot

atpH-atpI

LSC · 14466-15460 · 995 bp

Back to Gilbertiodendron

Pi 0.0206

MarkerSeek score 76.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 995
variable_sites: 29
pi: 0.0206
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AATAGAAGCAAGCCCGACAG	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.0	57.9	1054-1127 bp	1.0	84.8
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1020-1093 bp	1.0	84.8
atpH-atpI_p3	AATAGAAGCAAGCCCGACAG	TCCCTGTCATGTTCCTTGGA	50.0	50.0	58.0	58.3	1103-1176 bp	1.0	83.7
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	TCCCTGTCATGTTCCTTGGA	45.0	50.0	58.0	58.3	1069-1142 bp	1.0	83.7
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	1021-1094 bp	1.0	83.6