Hotspot

trnR-UCU-atpA

LSC · 10338-10774 · 437 bp

Back to Gilbertiodendron

Pi 0.0278

MarkerSeek score 70.60

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 437
variable_sites: 17
pi: 0.0278
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	CATTTACCGGGGAAGCAGAA	47.6	50.0	57.3	57.9	536-551 bp	1.0	70.5
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	AAGACATTTACCGGGGAAGC	47.6	50.0	57.3	57.9	540-555 bp	1.0	70.4
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	GGAAGCAGAAGCCCTTTTGA	47.6	50.0	57.3	58.4	526-541 bp	1.0	68.5
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	ATTTACCGGGGAAGCAGAAG	47.6	50.0	57.3	57.6	535-550 bp	1.0	68.1
trnR-UCU-atpA_p5	TGGATAGGACAGAGGTCTTCT	CATTTACCGGGGAAGCAGAA	47.6	50.0	56.9	57.9	571-586 bp	1.0	67.3