Hotspot

rpoC2

LSC · 26842-31368 · 4527 bp

Back to Gigantochloa

Pi 0.000707

MarkerSeek score 24.20

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4527
variable_sites: 9
pi: 0.000707
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 24.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	CTGGTCGAATTAGGGGAAGC	TCGATTCAACAAGGTGCCAA	55.0	45.0	58.0	58.0	2220-2253 bp	1.0	79.7
rpoC2_p2	CTGGTCGAATTAGGGGAAGC	TTCGATTCAACAAGGTGCCA	55.0	45.0	58.0	58.0	2221-2254 bp	1.0	79.7
rpoC2_p3	CAACCATTTCGAGCACAACC	TCGATTCAACAAGGTGCCAA	50.0	45.0	57.9	58.0	2325-2358 bp	1.0	79.7
rpoC2_p4	TTCGCTCCACAAGGATCAAG	ACCAATCCACCTAGTCGACT	50.0	50.0	57.9	57.8	1029-1062 bp	1.0	77.8
rpoC2_p5	TTCGCTCCACAAGGATCAAG	TTAGGGATGAGGATCTGGGC	50.0	55.0	57.9	58.3	953-986 bp	1.0	77.6