Hotspot

rpl16

LSC · 80064-81572 · 1509 bp

Back to Gigantochloa

Pi 0.00177

MarkerSeek score 44.60

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1509
variable_sites: 8
pi: 0.00177
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCCAGAAAGAAAAACCTGGT	AGCCTTATTTTTCCTGAAATCCC	40.0	39.1	54.7	57.1	1658-1666 bp	1.0	40.4
rpl16_p2	TCCAGAAAGAAAAACCTGGT	GCCTTATTTTTCCTGAAATCCCT	40.0	39.1	54.7	57.1	1657-1665 bp	1.0	40.4
rpl16_p3	TCCAGAAAGAAAAACCTGGT	TGACAAGAGCCTTATTTTTCCTG	40.0	39.1	54.7	57.0	1665-1673 bp	1.0	40.4
rpl16_p4	TCCAGAAAGAAAAACCTGGT	TCCTGAAATCCCTGAAAAAGAAGA	40.0	37.5	54.7	58.0	1647-1655 bp	1.0	40.4
rpl16_p5	TCCAGAAAGAAAAACCTGGTT	GCCTTATTTTTCCTGAAATCCCT	38.1	39.1	55.5	57.1	1657-1665 bp	1.0	40.4