Hotspot

clpP-psbB

LSC · 70611-71130 · 520 bp

Back to Gigantochloa

Pi 0.00192

MarkerSeek score 44.80

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 520
variable_sites: 3
pi: 0.00192
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 44.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	ACCCAAGTCGCTTCTTCATC	TATGCACCGAAAGCAATCGA	50.0	45.0	57.9	58.0	644-652 bp	1.0	80.5
clpP-psbB_p2	TCAACCCAAGTCGCTTCTTC	TATGCACCGAAAGCAATCGA	50.0	45.0	58.1	58.0	647-655 bp	1.0	80.4
clpP-psbB_p3	CTTTTGGAACACCAATGGGC	TATGCACCGAAAGCAATCGA	50.0	45.0	57.8	58.0	604-612 bp	1.0	80.1
clpP-psbB_p4	ACCCAAGTCGCTTCTTCATC	GGCCCAACCAGAAACTAGAG	50.0	55.0	57.9	57.9	676-684 bp	1.0	79.7
clpP-psbB_p5	TCAACCCAAGTCGCTTCTTC	GGCCCAACCAGAAACTAGAG	50.0	55.0	58.1	57.9	679-687 bp	1.0	79.5