Hotspot

rbcL-psaI

LSC · 58144-59390 · 1247 bp

Back to Gigantochloa

Pi 0.00214

MarkerSeek score 40.40

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1247
variable_sites: 6
pi: 0.00214
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	CGCAGCTTGTGAAGTATGGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.3	58.0	1384-1385 bp	1.0	77.9
rbcL-psaI_p2	CGCAGCTTGTGAAGTATGGA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.3	57.9	1390-1391 bp	1.0	77.3
rbcL-psaI_p3	GGCGATCAAATTCGAGTTCG	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.6	58.0	1363-1364 bp	1.0	77.3
rbcL-psaI_p4	TGGAAGGCGATCAAATTCGA	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.6	58.0	1368-1369 bp	1.0	77.0
rbcL-psaI_p5	GGCGATCAAATTCGAGTTCG	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.6	57.9	1369-1370 bp	1.0	76.7