Hotspot

trnT-UGU-trnL-UAA

LSC · 47965-48774 · 810 bp

Back to Gigantochloa

Pi 0.000741

MarkerSeek score 44.60

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 810
variable_sites: 1
pi: 0.000741
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	ATTTGAACCGATGACCCTCG	ACAATCAAGTCCGTAGCGTC	50.0	50.0	58.0	58.0	910-911 bp	1.0	80.4
trnT-UGU-trnL-UAA_p2	CGATGACCCTCGCATTACAA	ACAATCAAGTCCGTAGCGTC	50.0	50.0	58.1	58.0	902-903 bp	1.0	80.1
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	ACAATCAAGTCCGTAGCGTC	55.0	50.0	57.9	58.0	880-881 bp	1.0	79.6
trnT-UGU-trnL-UAA_p4	ATTTGAACCGATGACCCTCG	CCGTAGCGTCTACCGATTTC	50.0	55.0	58.0	58.2	900-901 bp	1.0	78.5
trnT-UGU-trnL-UAA_p5	ATTTGAACCGATGACCCTCG	CAATCAAGTCCGTAGCGTCT	50.0	50.0	58.0	57.7	909-910 bp	1.0	78.3