Hotspot

psbZ-trnfM-CAU

LSC · 12199-12989 · 791 bp

Back to Gigantochloa

Pi 0.00194
MarkerSeek score 44.60
Primer available yes
Label psbZ-trnfM-CAU

Feature Metadata

label_name
psbZ-trnfM-CAU
feature_type
igs
region
LSC
length_bp
791
variable_sites
4
pi
0.00194
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnfM-CAU_p1 TTGCTTCTCCTGATGGTTGG CGAGGCTCATAACCTTGAGG 50.0 55.0 57.8 57.8 948-955 bp 1.0 76.8
psbZ-trnfM-CAU_p2 GCTTCTCCTGATGGTTGGTC CGAGGCTCATAACCTTGAGG 55.0 55.0 58.3 57.8 946-953 bp 1.0 76.4
psbZ-trnfM-CAU_p3 TTGCTTCTCCTGATGGTTGG CAGTTTGGTAGCTCACGAGG 50.0 55.0 57.8 58.3 963-970 bp 1.0 76.4
psbZ-trnfM-CAU_p4 GCTTCTCCTGATGGTTGGTC CAGTTTGGTAGCTCACGAGG 55.0 55.0 58.3 58.3 961-968 bp 1.0 76.0
psbZ-trnfM-CAU_p5 TTGCTTCTCCTGATGGTTGG GAGCGGAGTAGAGCAGTTTG 50.0 55.0 57.8 58.4 976-983 bp 1.0 75.8