Hotspot

psbA

LSC · 308-1369 · 1062 bp

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Pi 0.00421
MarkerSeek score 34.10
Primer available yes
Label psbA

Feature Metadata

label_name
psbA
feature_type
gene
region
LSC
length_bp
1062
variable_sites
12
pi
0.00421
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
34.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA_p1 GATCCACTTGGCTACATCCG AACATCGATATCGGCGGATG 55.0 50.0 58.1 58.0 1438-1471 bp 1.0 84.4
psbA_p2 GATCCACTTGGCTACATCCG CATCGATATCGGCGGATGTT 55.0 50.0 58.1 58.0 1436-1469 bp 1.0 84.4
psbA_p3 CAATCCACTGCCTTGATCCA AACATCGATATCGGCGGATG 50.0 50.0 57.9 58.0 1452-1485 bp 1.0 84.3
psbA_p4 CAATCCACTGCCTTGATCCA CATCGATATCGGCGGATGTT 50.0 50.0 57.9 58.0 1450-1483 bp 1.0 84.3
psbA_p5 AATCCACTGCCTTGATCCAC CATCGATATCGGCGGATGTT 50.0 50.0 57.9 58.0 1449-1482 bp 1.0 84.3