Hotspot

ndhF

SSC · 111659-113908 · 2250 bp

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Pi 0.017
MarkerSeek score 55.00
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2250
variable_sites
91
pi
0.017
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 ACTTCCGGGACTAAACAGGA ACCAAGCCTAACTCTTCACC 50.0 50.0 58.0 57.1 2795-2919 bp 0.857143 74.3
ndhF_p2 GCAGAATACCGTCACCCATT ACCAAGCCTAACTCTTCACC 50.0 50.0 58.0 57.1 2875-2999 bp 0.857143 74.1
ndhF_p3 ACAGGAACAAGAGGGATCCA ACCAAGCCTAACTCTTCACC 50.0 50.0 58.0 57.1 2781-2905 bp 0.857143 74.1
ndhF_p4 AAGAGGGATCCACCGAAGAA ACCAAGCCTAACTCTTCACC 50.0 50.0 58.0 57.1 2773-2897 bp 0.857143 74.1
ndhF_p5 GAAGATCCTTCCCCTTCCCT ACCAAGCCTAACTCTTCACC 55.0 50.0 58.1 57.1 2756-2880 bp 0.857143 73.7