Hotspot

ndhA

SSC · 121267-123562 · 2296 bp

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Pi 0.0141
MarkerSeek score 50.20
Primer available yes
Label ndhA

Feature Metadata

label_name
ndhA
feature_type
gene
region
SSC
length_bp
2296
variable_sites
77
pi
0.0141
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA_p1 CCCGTACTGTTTGTTGACCA AGAGTTGAAGCCCCAAAAGG 50.0 50.0 58.0 58.0 2588-2634 bp 1.0 84.1
ndhA_p2 CCCGTACTGTTTGTTGACCA GTGAGAGTTGAAGCCCCAAA 50.0 50.0 58.0 58.0 2591-2637 bp 1.0 84.0
ndhA_p3 CCCGTACTGTTTGTTGACCA AGTTGAAGCCCCAAAAGGAG 50.0 50.0 58.0 58.0 2586-2616 bp 0.571429 66.1
ndhA_p4 CCCGTACTGTTTGTTGACCA GAGTTGAAGCCCCAAAAGGA 50.0 50.0 58.0 58.0 2587-2617 bp 0.571429 66.0
ndhA_p5 CCCGTACTGTTTGTTGACCA GTTGAAGCCCCAAAAGGAGA 50.0 50.0 58.0 58.0 2585-2615 bp 0.571429 66.0