Hotspot

rps15-ycf1

SSC · 125121-125533 · 413 bp

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Pi 0.0354
MarkerSeek score 67.40
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
413
variable_sites
37
pi
0.0354
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.96
markerseek_score
67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 CAGCTGTTCCGGTCCATTTA GGATGCATATGTATCCGCGA 50.0 50.0 57.9 57.7 1069-1093 bp 1.0 82.5
rps15-ycf1_p2 AGCTGTTCCGGTCCATTTAC GGATGCATATGTATCCGCGA 50.0 50.0 57.9 57.7 1068-1092 bp 1.0 82.5
rps15-ycf1_p3 AAGATAGCAATCGTTGGCGT GGATGCATATGTATCCGCGA 45.0 50.0 58.0 57.7 628-647 bp 0.857143 78.8
rps15-ycf1_p4 AGATAGCAATCGTTGGCGTT GGATGCATATGTATCCGCGA 45.0 50.0 58.0 57.7 627-646 bp 0.857143 78.8
rps15-ycf1_p5 AGATAGCAATCGTTGGCGTT ATCGCCTAGACTTCGGAGAG 45.0 55.0 58.0 58.4 887-906 bp 0.714286 68.3