Hotspot

ndhF-rpl32

SSC · 113909-114995 · 1087 bp

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Pi 0.0239
MarkerSeek score 64.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1087
variable_sites
72
pi
0.0239
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AGACATGCAGGGGAAATTGG CGAATCAAGCTGATGGGGAA 50.0 50.0 58.1 58.0 2170-2218 bp 1.0 84.6
ndhF-rpl32_p2 CCAGACATGCAGGGGAAATT CGAATCAAGCTGATGGGGAA 50.0 50.0 58.1 58.0 2172-2220 bp 1.0 84.6
ndhF-rpl32_p3 ATTGGGCGGATTTAGCAACT CGAATCAAGCTGATGGGGAA 45.0 50.0 57.9 58.0 2155-2203 bp 1.0 84.6
ndhF-rpl32_p4 CCCACATACGACGAAGGTTT CGAATCAAGCTGATGGGGAA 50.0 50.0 57.9 58.0 1561-1594 bp 0.857143 77.8
ndhF-rpl32_p5 CCCACATACGACGAAGGTTT AGACGCTCAAATTGATGCCT 50.0 45.0 57.9 57.9 1865-1896 bp 0.857143 77.3