Hotspot

psbE-petL

LSC · 65376-66598 · 1223 bp

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Pi 0.0179
MarkerSeek score 62.20
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1223
variable_sites
58
pi
0.0179
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 AGAACGTTCTCCTGTGCTTC ACCGAAATAACTAGTTATAGTAGGCA 50.0 34.6 57.8 57.5 1267-1287 bp 1.0 45.3
psbE-petL_p2 GTTCTCCTGTGCTTCCAGAC ACCGAAATAACTAGTTATAGTAGGCA 55.0 34.6 58.2 57.5 1262-1282 bp 1.0 45.3
psbE-petL_p3 TCTCCTGTGCTTCCAGACAT ACCGAAATAACTAGTTATAGTAGGCA 50.0 34.6 58.4 57.5 1260-1280 bp 1.0 45.3
psbE-petL_p4 ACGAATAACCAACCCGCAAT ACCGAAATAACTAGTTATAGTAGGCA 45.0 34.6 58.2 57.5 1353-1373 bp 1.0 44.9
psbE-petL_p5 TGCTGACGAATAACCAACCC ACCGAAATAACTAGTTATAGTAGGCA 50.0 34.6 58.2 57.5 1358-1378 bp 1.0 44.9