Hotspot

psbZ-trnG-GCC

LSC · 36761-37198 · 438 bp

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Pi 0.0432
MarkerSeek score 73.60
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
438
variable_sites
44
pi
0.0432
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 AATGACCCTCGATTCCATGC CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.0 58.0 1088-1163 bp 1.0 87.0
psbZ-trnG-GCC_p2 AATGACCCTCGATTCCATGC AATGCGATGCGGTCAACTAT 50.0 45.0 58.0 58.0 958-1039 bp 1.0 86.4
psbZ-trnG-GCC_p3 AATGACCCTCGATTCCATGC CGTTGTTTTTCGACCGGAAG 50.0 50.0 58.0 58.0 1416-1491 bp 1.0 86.0
psbZ-trnG-GCC_p4 GAATAAGGCGGTAAGGGGTG CGTTGTTTTTCGACCGGAAG 55.0 50.0 58.0 58.0 1394-1403 bp 0.714286 71.1
psbZ-trnG-GCC_p5 AATGACCCTCGATTCCATGC ATAATGCGATGCGGTCAACT 50.0 45.0 58.0 58.0 961-962 bp 0.285714 51.0