Hotspot

trnR-UCU-atpA

LSC · 10281-10910 · 630 bp

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Pi 0.0351
MarkerSeek score 71.60
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
630
variable_sites
48
pi
0.0351
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 AGGAGTGAAAAGCGTCCATT TGGAACGTTTTCTACTTCAGGA 45.0 40.9 57.4 57.3 745-777 bp 1.0 63.1
trnR-UCU-atpA_p2 GGAGTGAAAAGCGTCCATTG TGGAACGTTTTCTACTTCAGGA 50.0 40.9 57.4 57.3 744-776 bp 1.0 62.7
trnR-UCU-atpA_p3 AAAGGAGTGAAAAGCGTCCA TGGAACGTTTTCTACTTCAGGA 45.0 40.9 57.7 57.3 747-776 bp 0.857143 57.6
trnR-UCU-atpA_p4 AAGGAGTGAAAAGCGTCCAT TGGAACGTTTTCTACTTCAGGA 45.0 40.9 57.4 57.3 746-775 bp 0.857143 55.8
trnR-UCU-atpA_p5 AAAGGAGTGAAAAGCGTCCA TGGAACGTTTTCTACTTCAGGAA 45.0 39.1 57.7 58.0 747-776 bp 0.857143 54.8